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      <JournalTitle>Journal of Medical and Surgical Research</JournalTitle>
      <Volume-Issue>Vol. VI, n 3</Volume-Issue>
      <Season>February 2020</Season>
      <ArticleType>JMSR Pathogens</ArticleType>
      <ArticleTitle>Detection of Mycobacterium tuberculosis Complex by Real-time PCR in Pericardial Fluid at Ibn Rochd University Hospital - Casablanca: A case report</ArticleTitle>
      <Abstract>The diagnosis of Pericardial tuberculosis may be difficult and elusive. Rapid treatment can be lifesaving but requires an accurate diagnosis. Conventional diagnostic methods are time-consuming and have low sensitivity, making bacteriological confirmation of the disease very difficult. Hereby, we report a case of a 27-year-old man who presented dyspnea with physical deterioration and anterior chest pain. The use of Real-time PCR for M. tuberculosis complex detection confirmed pericardial tuberculosis. The patient had an excellent response to a three-drug combination anti-tuberculous regimen and 1 year later was asymptomatic, without evidence of constrictive pericarditis.</Abstract>
      <Keywords>IS6110, MTBC, Pericardial tuberculosis, RT-PCR</Keywords>
        <Abstract>https://journal-jmsr.net/ubijournal-v1copy/journals/abstract.php?article_id=7172&amp;title=Detection of Mycobacterium tuberculosis Complex by Real-time PCR in Pericardial Fluid &#13;
at Ibn Rochd University Hospital - Casablanca: A case report</Abstract>
        <References>Flego V, Lender DM, Bulat-Kardum L. Pericardial effusion as the first manifestation of occupational tuberculosis in a health care worker. Archives of Industrial Hygiene and Toxicology. 2014;65(4):417–422.&#13;
	Nabih O, Bouzoubaa S and Benyahya I., “Oral Localization of Tuberculosis?: Case Report and Literature Review,” Journal of Medical and Surgical Research (J Med Sur Res) 2015; 4(1). 373–376.&#13;
	Alami F, El Merrouni S, Oudbib M, Abdellah E, Berraho A. Systemic Tuberculosis Revealed by Ophthalmic Manifestations. Journal of Medical and Surgical Research (J Med Sur Res) 2015; 2(2): 161- 162..&#13;
	Cock KMD. Tuberculosis: Pathogenesis, protection, and control: B. R. Bloom (editor). Washington, DC: American Society for Microbiology, Transactions of the Royal Society of Tropical Medicine and Hygiene. 1995; 89(5):579–80.&#13;
	Heart Inflammation | National Heart, Lung, and Blood Institute (NHLBI). https://www.nhlbi.nih.gov/health-topics/heart-inflammation#Signs,-Symptoms,-and-Complications (accessed 1 Oct2019).&#13;
	Hopewell PC. Overview of Clinical Tuberculosis. Tuberculosis. 1994;:25–46.&#13;
	Bourkadi P. J., “Management of Tuberculosis: What Have Changed?” Journal of Medical and Surgical Research (J Med Sur Res) 2015; 2 (2), 139.&#13;
	Tzoanopoulos D, Stakos D, Hatseras D, Ritis K, Kartalis G. Detection of Mycobacterium tuberculosis complex DNA in pericardial fluid, bone marrow and peripheral blood in a patient with pericardial tuberculosis. A case report. Neth J Med. 2001; 59(4):177–80.&#13;
	Rana BS, Jones RA, Simpson IA. Recurrent pericardial effusion: the value of polymerase chain reaction in the diagnosis of tuberculosis. Heart. 1999; 82(2):246–7.&#13;
	Seino Y, Ikeda U, Kawaguchi K, et al. Tuberculous pericarditis presumably diagnosed by polymerase chain reaction analysis. Am Heart J. 1993;126(1):249–51.&#13;
	Barletta F, Vandelannoote K, Collantes J, et al. Standardization of a TaqMan-based real-time PCR for the detection of Mycobacterium tuberculosis-complex in human sputum. Am J Trop Med Hyg. 2014;91(4):709–14.&#13;
	Sanduzzi A, Ponticiello A, Bocchino M et al. Latent tuberculosis infection (LTBI): a real host defence or a permanent threat? Infez Med. 2016;24(3):179–82.&#13;
	Tavares C, Lins TBA, Junqueira-Kipnis AP, et al. Tuberculosis deaths in a tertiary hospital in Goiand;acirc;nia, Brazil: a descriptive study. Infez Med. 2013;21(4):279–86.&#13;
	Lakhal Z, Benaich F, Mouine N, et al. Acute Purulent Tuberculosis Pericarditis with Cardiac Tamponade: a Case Report. Journal of Medical and Surgical Research. 2015;2(2) :157–160.&#13;
	Reuter H, Burgess L, van Vuuren W, et al. Diagnosing tuberculous pericarditis. QJM. 2006;99(12):827–39.&#13;
	WHO | Tuberculosis country profiles. WHO. http://www.who.int/tb/country/data/profiles/en/ (accessed 8 Jul2018).&#13;
	Metaxas EI, Kotsifas K, Tatsis G, et al. Tuberculous pericarditis: three cases and brief review. Monaldi Arch Chest Dis. 2010;73(1):44–50.&#13;
	Tzoanopoulos D, Mimidis K, Giaglis S, et al. . The usefulness of PCR amplification of the IS6110 insertion element of M. tuberculosis complex in ascitic fluid of patients with peritoneal tuberculosis. Eur J Intern Med. 2003;14(6):367–71.&#13;
	Ritis K, Tzoanopoulos D, Speletas M et al.,. Amplification of IS6110 sequence for detection of Mycobacterium tuberculosis complex in HIV-negative patients with fever of unknown origin (FUO) and evidence of extrapulmonary disease.J Intern Med. 2000;248(5):415–424</References>