JOURNAL OF MEDICAL AND SURGICAL RESEARCH - Special Issue "Pathology", March, 2023

Pages: 1151-1151
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Ret rearrangement in papillary thyroid carcinoma.

Author: Fidélia Nihad Da-Silva, Sanaa Hachimi, Meriem Regragui, Asmae Mazti, Samira Benayad, Nisrine Bennani-Guebessi, Farida Marnissi, Mehdi Karkouri

Category: JMSR Pathology

Abstract:

Introduction: Papillary thyroid carcinoma accounts for 80% of all cases. The only documented risk factor for PTC is exposure to ionizing radiation. The prognosis is generally excellent. During the last 2 decades numerous studies on genetic alterations have demonstrated the pathogenic role of RET (rearranged during transfection) proto-oncogene rearrangements in PTC.

Our work aims to investigate RET rearrangements in a series of papillary thyroid carcinoma.

Materials and methods: This is a series of randomly selected cases of histological papillary thyroid carcinoma diagnosed at the laboratory of anatomy and cytological pathology of the CHU Ibn Rochd of Casablanca. To evaluate RET rearrangements, we performed FISH analysis on formalin-fixed and paraffin-embedded tissues using a RET gene break apart probe reagent.

Results : A total of 12 cases of papillary carcinoma were collected. The average age of our patients was 48.8 years and all were female. The tumor size was variable with extremes between 15mm and 55mm long axis, and multifocal in 5 cases (41.7%).

Among the histological subtypes, the vesicular and classical variants were the most frequent. Indeed, 4 cases (33.3%) of the vesicular variant and 4 cases (33.3%) of the classical variant were found, followed by the high cell variant in 2 cases (17%). Finally, the oncocytic variant was present in 1 case (8.7%) and the combination of vesicular and oncocytic variants also in 1 case (8.7%). Vascular emboli were present in 83.3% (10 cases) and extra-thyroidal extension in 5 cases. RET rearrangement was observed in 2 cases (16.7%) whose epidemiological and histological characteristics are described in the table.

Conclusion: The search for molecular alterations in iodine-refractory thyroid carcinoma is desirable especially in view of the availability of systemic treatments and targeted therapies with promising results.

Keywords: Papillary Thyroid Carcinoma, RET Rearrangement, Targeted Therapy

Full Text:

Ret rearrangement in papillary thyroid carcinoma

Fidélia Nihad Da-Silva, Sanaa Hachimi, Meriem Regragui, Asmae Mazti, Samira Benayad, Nisrine Bennani-Guebessi, Farida Marnissi, Mehdi Karkouri

Department of anatomical pathology, Ibn Rochd University Hospital Center, Hassan II University of Casablanca, Faculty of Medicine and Pharmacy, Casablanca, Morocco

Introduction: Papillary thyroid carcinoma accounts for 80% of all cases. The only documented risk factor for PTC is exposure to ionizing radiation. The prognosis is generally excellent. During the last 2 decades numerous studies on genetic alterations have demonstrated the pathogenic role of RET (rearranged during transfection) proto-oncogene rearrangements in PTC.

Our work aims to investigate RET rearrangements in a series of papillary thyroid carcinoma.

Materials and methods: This is a series of randomly selected cases of histological papillary thyroid carcinoma diagnosed at the laboratory of anatomy and cytological pathology of the CHU Ibn Rochd of Casablanca. To evaluate RET rearrangements, we performed FISH analysis on formalin-fixed and paraffin-embedded tissues using a RET gene break apart probe reagent.

Results : A total of 12 cases of papillary carcinoma were collected. The average age of our patients was 48.8 years and all were female. The tumor size was variable with extremes between 15mm and 55mm long axis, and multifocal in 5 cases (41.7%).

Among the histological subtypes, the vesicular and classical variants were the most frequent. Indeed, 4 cases (33.3%) of the vesicular variant and 4 cases (33.3%) of the classical variant were found, followed by the high cell variant in 2 cases (17%). Finally, the oncocytic variant was present in 1 case (8.7%) and the combination of vesicular and oncocytic variants also in 1 case (8.7%). Vascular emboli were present in 83.3% (10 cases) and extra-thyroidal extension in 5 cases. RET rearrangement was observed in 2 cases (16.7%) whose epidemiological and histological characteristics are described in the table.

Conclusion: The search for molecular alterations in iodine-refractory thyroid carcinoma is desirable especially in view of the availability of systemic treatments and targeted therapies with promising results.

Keywords : Papillary Thyroid Carcinoma, RET Rearrangement, Targeted Therapy.

Conflict of interest statement : None

References:

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